Figure 10.

Rescue of syntaxin-1 localization upon reintroduction of F115E/E132A mutant Munc18-1-EmGFP into Munc18-1/-2 double knockdown clones. Confocal images of D7 cells that were infected with lentiviruses that express F115E/E132A Munc18-1-EmGFP (left) and stained with anti-syntaxin1 antibodies followed by red-x-conjugated anti-mouse antibodies (middle). Right panels are merged pictures. The existence of perinuclear localization of syntaxin-1 in the presence of its apparent plasma membrane localization was apparent in top panels but not in bottom panels. Bar, 10 μm.