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. 2009 Dec 1;20(23):4962–4975. doi: 10.1091/mbc.E09-08-0712

Table 1.

Thermodynamic parameters for the binding of syntaxin-1A (Sx1a, residues 2-243) to Munc18-1 determined by ITC

Proteins interaction ΔH (kCal mol−1) TΔS (kCal mol−1) ΔG (kCal mol−1) Kd (nM) n
Munc18-1-WT:Sx1a2-243 −20.6 ± 1.05 −10.1 ± 1.08 −10.5 ± 0.10 22.8 ± 3.94 1.04 ± 0.02
Munc18-1-F115E:Sx1a2-243 −14.3 ± 2.01* −3.86 ± 2.01* −10.4 ± 0.04 23.1 ± 1.42 0.96 ± 0.03
Munc18-1-E132A:Sx1a2-243 −15.5 ± 1.40* −5.31 ± 1.41* −10.1 ± 0.03* 35.8 ± 1.20 1.05 ± 0.02

Values are expressed as means ± SEM (Munc18-1-WT, n = 6; Munc18-1-F115E or Munc18-1-E132A, n = 3). All experiments were performed at 298 K.

Significant at *p < 0.05.