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. 2009 Dec 1;20(23):4976–4984. doi: 10.1091/mbc.E09-04-0295

Figure 7.

Figure 7.

Endogenous HSF1 immunoprecipitates with endogenous HDAC1 and 2. Immunoprecipitations anti HDAC1 and HDAC2 were performed with anti HDAC1 and 2. Equal amounts of HDAC1 and 2 and HSF1 in non-heat-shocked and heat-shocked extracts was controlled by Western blot in total cell extracts (Input). Immunoprecipitations were performed on cellular extracts from unstressed (−) and heat-shocked (+) cells. Equal level of HDAC1 and 2 in the immunoprecipitates was also controlled by Western blot. The presence of HSF1 in the immunoprecipitates from heat-shocked cells was determined by Western blot with anti-HSF1 (the arrow indicates the signal corresponding to HSF1). Immunoprecipitations were also performed with nonspecific rabbit IgG used as controls of specificity (Ctl).