Figure 1.

BCH1 and BUD7 are redundantly required for Fus1p plasma membrane localization. (A) Fus1p-GFP localization in (a) wild-type, (b) chs5Δ, (c) bch2Δ chs6Δ bud7Δ, (d) bch1Δ, and (e) bch1Δ bud7Δ cells. All cells were also mutant for BAR1. Cells were treated with α-factor for 90 min and then visualized by fluorescence microscopy. (B) Fus1p-Myc protease accessibility in exomer mutants. Asterisk (*) indicates endogenously cleaved Fus1p-Myc and exogenously cleaved Fus1p-Myc (see text for details). Quantifications are the average of five or more independent experiments. Error bars represent the 95% confidence interval.