Figure 2.

AP-1 is important for the intracellular retention of Fus1p in chs5Δ and bch1Δ bud7Δ mutants. (A) Fus1p-GFP localization in (a) wild-type, (b) chs5Δ, (c) chs5Δ apl2Δ, (d) bch1Δ bud7Δ, and (e) bch1Δ bud7Δ apl2Δ cells. All cells were also mutant for BAR1. Cells were treated with α-factor for 90 min and then visualized by fluorescence microscopy. (B) Fus1p-Myc protease accessibility in exomer AP-1 mutants. Asterisk (*) indicates endogenously cleaved Fus1p-Myc and exogenously cleaved Fus1p-Myc (see text for details). Quantifications are the average of three or more independent experiments. Error bars represent the 95% confidence interval.