Table 1.
S. cerevisiae strains used in this study
| Name | Genotype | Source |
|---|---|---|
| PJ69-4A | MATa trp1-901 leu2-3,112 ura3-52 his3-200 gal4D gal80D LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ | James et al. (1996) |
| YPH499 | MATa ade2-101oc his3-Δ 200 leu2-Δ1 lys2-801am trp1-Δ 63 ura3-52 | Sikorski and Hieter (1989) |
| YPH500 | MATα ade2-101oc his3-Δ 200 leu2-Δ1 lys2-801am trp1-Δ 63 ura3-52 | Sikorski and Hieter (1989) |
| YAS9 | MATa YPH499 bar1Δ::KANMX6 | J. Thorner, University of California–Berkeley |
| YRB38 | MATa YAS9 FUS1-13Myc::TRP1 | This study |
| YRB41 | MATa YAS9 bch1Δ::HIS3 | This study |
| YRB46 | MATa YPH499 bar1Δ::HIS3 bud7Δ::KANMX4 | This study |
| YRB48 | MATa YAS9 chs6Δ::HIS3 | This study |
| YRB56 | MATa YRB41 bch2Δ::LEU2 | This study |
| YRB57 | MATa YRB41 chs6Δ::TRP1 | This study |
| YRB86 | MATa YRB46 bch1Δ::LEU2 | This study |
| YRB90 | MATa YAS9 chs5Δ::HIS3 | This study |
| YRB100 | MATa YRB41 FUS1-13Myc::TRP1 | This study |
| YRB103 | MATa YRB86 FUS1-13Myc::TRP1 | This study |
| YRB106 | MATa YRB90 FUS1-13Myc::TRP | This study |
| YRB132 | MATa YPH499 bar1Δ::HIS3 bud7Δ::KANMX4 bch1Δ::LEU2 apl2Δ::TRP1 | This study |
| YRB151 | MATa YPH499 bar1Δ::HIS3 bud7Δ::KANMX4 bch1Δ::LEU2 apl2Δ::URA3 FUS1-13Myc::TRP1 | This study |
| YRB168 | MATa YPH499 bar1Δ::HIS3 bch2 Δ::URA3 bud7 Δ::KANMX4 chs6 Δ::LEU2 FUS1-13Myc::TRP1 | This study |
| YRB171 | MATa YPH499 bar1Δ::HIS3 bch2Δ::NATMX bud7Δ::KANMX4 chs6Δ::LEU2 | This study |
| YRB180 | MATa YPH499 bar1Δ::HIS3 bch1Δ::LEU2 bch2Δ::URA3 bud7Δ::KANMX4 chs6Δ::HIS3 FUS1-13Myc::TRP1 | This study |
| YRB191 | MATa YPH499 bar1Δ::HIS3 fus1Δ::KANMX4 | This study |
| YRB192 | MATa YPH499 fus1Δ::KANMX4 | This study |
| YRB193 | MATα YPH500 fus1Δ::KANMX4 | This study |
| YRB202 | MATa YPH499 chs5Δ::LEU2 | This study |
| YRB207 | MATa YRB90 apl2Δ::TRP2 | This study |
| YRB208 | MATa YRB106 apl2Δ::URA3 | This study |
| YRB209 | MATa YPH499 FUS1-13Myc::TRP1 | This study |
| YRB210 | MATa YPH499 bud7Δ::KANMX4 FUS1-13Myc::TRP1 | This study |
| YRB211 | MATa YPH499 bch1Δ::LEU2 FUS1-13Myc::TRP1 | This study |
| YRB212 | MATa YPH499 bch1Δ::LEU2 bud7Δ::KANMX4 | This study |
| YRB213 | MATa YRB202 apl2Δ::URA3 | This study |
| YRB214 | MATa YRB212 apl2Δ::URA3 | This study |
| YRB215 | MATa YRB191 apl2Δ::TRP2 | This study |
| YRB216 | MATa YRB192 apl2Δ::TRP2 | This study |
| YRB217 | MATa YRB191 chs5Δ::TRP2 | This study |
| YRB218 | MATa YRB191 SEC7-RFP::TRP | This study |
| YSS27 | MATa leu2 ura3-52 trp1 prb1-1122 pep4-3 pre1-451 CHS5-Stag-TEV-ZZ::KanMX | Sanchatjate and Schekman (2006) |