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. 2009 Dec 1;20(23):5007–5025. doi: 10.1091/mbc.E09-06-0490

Figure 3.

Figure 3.

Depletion of Utp10p, a member of the Utp8p-containing subcomplex, or Utp13p, a Utp protein involved in 18S rRNA biogenesis does not affect nuclear tRNA export. The wild-type BY4741 strain harboring pCEN-URA-GAL1 (A), utp8 pCEN-URA-GAL1-UTP8 (B), utp10 pCEN-URA-GAL1-UTP10 (C), and utp13 BG1805-CEN-URA-GAL1-UTP13 (D) were grown in CS medium containing 2% galactose and lacking uracil to A600 of 0.6 at 30°C. The cells were washed and grown in CS medium containing 2% dextrose and lacking uracil at 30°C for 6 h. FISH was then used to monitor the nuclear-cytoplasmic distribution of tRNATyr. DNA was visualized by DAPI staining.