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Nutrient stress blocks nuclear export of retrograded mature tRNA obtained from intron-containing pre-tRNAs, but not export of mature tRNA made from intronless precursor. S. cerevisiae cells were starved of amino acids (top panels) or nitrogen (bottom panels) in synthetic medium containing glucose for the times indicated, and the cellular distribution of mature tRNATyr (left panels) and tRNAGly (right panels) was monitored by FISH. The DNA was visualized by DAPI staining.
