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Heat inactivation of rePON1 and huPON1. Purified enzymes diluted in TBS with 1 mM CaCl2 were incubated at the range of temperatures (25 - 80°C) for 30 mins, and residual PON1 activity was determined. Data were fitted to the sigmoidal decay function to give Tm values of 47.2°C for huPON1 and 60.4°C for rePON1 (the corresponding m values are 0.273 and 0.399; see Methods). Each point represents mean ± SD of 3 experiments.
