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Inactivation of recombinant and human PON1, and PON1-HDL complexes by tetranitromethane. PON1 samples were incubated with 1 mM of TNM at 37°C. Residual PON1 activity was determined after 0.25 and 0.5 hrs of incubation, and plotted as percentage of the initial activity. Residual PON1 activity with no addition of TNM remained unchanged for all the samples (~100%). Data represents mean ± SD of 3 independent experiments performed in duplicates.
