Table 1.
representative results of detoxification studies
| Time prior CPO induction | Group1 | Score | Mortality (%) |
Severe reaction (%) |
|---|---|---|---|---|
| untreated2 | 3.6 | 62.5 | 37.5 | |
| TBS | 3.8 | 75 | 25 | |
| POPC | 3.9 | 87.5 | 12.5 | |
| 5 min | ||||
| Atropine+2PAM3 | 0.5* | 0 | 0 | |
| BL-30504 | 1.3* | 12.5 | 0 | |
| 3 hours | ||||
| Atropine+2PAM3 | 2.1 | 25 | 12.5 | |
| BL-30504 | 1.3* | 12.5 | 0 | |
| rePON1-TBS4 | 1.4* | 12.5 | 12.5 | |
| 14 hours | ||||
| BL-30504 | 3.0 | 50 | 25 | |
| rePON1-TBS4 | 1.8* | 0 | 12.5 | |
| rePON1-TBS5 | 1.1* | 12.5 | 12.5 | |
Single oral gavage of CPO (23 mg/kg, 10 mL/kg dose) to male C57BL/6J mice was preceded (5 min, 3 hr or 14 hrs prior to CPO intoxication) by no treatment or by injection of TBS (buffer only), POPC (2.5 mg/animal), rePON1 (0.4 mg and 0.63 mg/animal), BL-3050 (0.4 mg rePON1/animal), and atropine (20 mg/kg) with 2-PAM (25 mg/kg). Animals were observed closely for clinical signs during the first 24 hours following CPO intoxication.
* - Significant difference (p < 0.05) compared to all control groups using Mann-Whitney test (see Methods and Results and Discussion sections for more details).
1 - All groups comprised of 8 mice
2 - Mice were only poisoned by CPO
3 - Atropine and 2-PAM concentrations were 20 mg/kg and 25 mg/kg, respectively
4 - Protein estimation amount for rePON1 was 0.4 mg/animal.
5 - Protein estimation amount for rePON1 was 0.63 mg/animal.