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siRNAs specific for GCH1 and their gene silencing effect. In the presence of rGCH1 expression vector (pcDNA3-rGCH1), 293T cells were transfected either scrambled control or rGCH1-specific various siRNAs. Two days after transfection, cells were harvested and western-blotting was carried out. The data revealed a significant decrease in the rGCH1 level is observed when siRNAs for rGCH1 are present.
