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Inhibition of microglial activation via rAAV-shGCH1 in the spinal cord dorsal horn. Nissl-staining was carried out in the frozen sections of spinal cords. Microglia in the spinal cord dorsal horn were visualized with the Iba-1 primary antibody on day 14 after either pain surgery for pre-treatment and virus administration for post-treatment. Immunohistochemistry data reveal that the number of Iba-1 positive cells increased in the rAAV-GFP pain group only. Scale bar = 100 μm.
