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Schematic representation of the p35:Nef and p35:AMCV-P19 constructs. In p35:Nef and p35:AMCV-P19 constructs, nef and amcv-p19 genes are under the control of the CaMV 35S promoter (35S) and the TMV 5'-untranslated leader sequence omega (Ω). nef: cDNA encoding a mutated form of Nef in which the myristoylation consensus sequence is abolished; amcv-p19: cDNA encoding AMCV-P19 gene silencing suppressor; tags: Flag-tag followed by a 6× histidine tag.
