Figure 3.

RRV ORF59 mRNA expression after RRV infection of RPFF cells. Near confluent cultures of RPFF cells were infected with RRV and incubated for various times. Levels of mRNA expressed by the RRV genes orf59 DNA polymerase processivity factor, orf50 transactivator, orf8 glycoprotein B, orf73 latency-associated nuclear antigen and orf9 DNA polymerase were determined by RT-qPCR as described in Materials and Methods. Viral mRNA levels were normalized to mRNA levels of the cellular ribosomal phosphoprotein (RPO) and expressed as a relative expression ratio.