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. 2009 Nov 18;6:205. doi: 10.1186/1743-422X-6-205

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Copyright ©2009 Bruce et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The 425 rabbit anti-RV2 ORF59 antiserum specifically recognizes the ORF59 proteins from the RV2 rhadinoviruses of three macaque species. Recombinant C-terminal polypeptides that were highly conserved between RRV and MneRV2 (see underlined antigenic sequences in Fig. 1) were expressed in bacteria with a 6XHis tag, purified and used to immunize a rabbit (425). Full length recombinant ORF59 proteins from RRV (Lane 1), RFHVMm (Lane 2), MneRV2 (Lane 3), RFHVMn (Lane 4), PtrRV2 (Lane 5), PtrRV1 (Lane 6) and KSHV (Lane 7) were expressed as glutathione synthase tranferase (GST) fusions, analyzed by SDS-PAGE and probed using either A) 425 rabbit anti-RV2 ORF59 antiserum, or B) anti-GST antiserum, as described in the Materials and Methods.