Table 3.
Dopaminergic | Norepinergic | Serotonergic | ||||
---|---|---|---|---|---|---|
Monoamine neurotransmitters (ng/mg of tissue) |
DA | HVA | NE | NM | 5-HT | 5-HIAA |
Prefrontal Cortex | ||||||
WT (n = 9) | 0.041 ± 0.028 | 0.102 ± 0.006 | 0.48 ± 0.018 | 0.077 ± 0.003 | 0.426 ± 0.021 | 0.238 ± 0.01 |
KO (n = 9) | 0.041 ± 0.018 | 0.086 ± 0.004* | 0.47 ± 0.017 | 0.083 ± 0.006 | 0.394 ± 0.017 | 0.25 ± 0.007 |
Hippocampus | ||||||
WT (n = 9) | 0.018 ± 0.001 | 0.038 ± 0.003 | 0.651 ± 0.023 | 0.056 ± 0.003 | 0.669 ± 0.019 | 0.705 ± 0.042 |
KO (n = 9) | 0.015 ± 0.002 | 0.041 ± 0.003 | 0.566 ± 0.016** | 0.051 ± 0.004 | 0.625 ± 0.016 | 0.747 ± 0.029 |
Amygdala | ||||||
WT (n = 9) | 0.805 ± 0.041 | 0.303 ± 0.013 | 0.498 ± 0.018 | 0.055 ± 0.003 | 0.562 ± 0.021 | 0.466 ± 0.031 |
KO (n = 9) | 0.835 ± 0.087 | 0.291 ± 0.012 | 0.506 ± 0.013 | 0.064 ± 0.003* | 0.523 ± 0.021 | 0.492 ± 0.021 |
Striatum | ||||||
WT (n = 9) | 13.49 ± 0.42 | 1.486 ± 0.049 | 0.12 ± 0.009 | 0.029 ± 0.003 | 0.425 ± 0.023 | 0.407 ± 0.028 |
KO (n = 9) | 13.486 ± 0.28 | 1.534 ± 0.036 | 0.117 ± 0.006 | 0.024 ± 0.002 | 0.42 ± 0.017 | 0.399 ± 0.01 |
Levels of monoamines and metabolites, including dopamine (DA), 4-hydroxy-3- methoxyphenylacetic acid (HVA), 3 norepinephrine (NE), normetanephrine (NM), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA), were quantified by HPLC with electrochemical detection in prefrontal cortex (PFC), hippocampus (HIP), amygdala (AM) and striatum (STR) of GSK-3α KO (n = 9) and WT (n = 9) mice. In bold type *p.value ≤ 0.05; **p.value ≤ 0.01 KO vs. WT.