Figure 3.
Residual dipolar coupling analysis of Fis1ΔTM at 14.2 T, 25 °C, and pH 7.4. (a) RDC values calculated from modified PDB files plotted vs measured values at pH 7.4. The reported Q value shows a poor correlation with the previously reported structure. (b) The differences between measured and calculated RDC values are plotted as a function of residue number to highlight the structural regions that give rise to the discrepancies in Q values with the reported structure. The largest differences between the structure and measured RDC values at pH 7.4 lie in the arm, helix 1, and helix 6.