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. Author manuscript; available in PMC: 2010 Sep 29.
Published in final edited form as: Biochemistry. 2009 Sep 29;48(38):9156–9169. doi: 10.1021/bi900757q

Figure 2.

Figure 2

Effect of thioredoxin deletion on p53 activity in thioredoxin reductase null yeast. For each transformation group, six independent transformants were assayed for β-galactosidase activity. Bars represent activity levels (mean ± S.D.), normalized to the level in wild-type yeast expressing native p53. Asterisk (*) indicates genotypes where p53 activity in wild-type and mutant yeast differed significantly (p < 0.05, by t-test). A. Effect of deleting thioredoxin on p53-dependent reporter gene expression in thioredoxin reductase null cells. Strains with the indicated genotypes were co-transformed with the pRS314PGK-p53 effector and pRS316-p53RE-LacZ reporter plasmids. B. Levels of p53-dependent reporter gene expression in yeast inheriting different combinations of the Δtrr1, Δtrx1 and Δtrx2 null alleles. A diploid that was heterozygous for null mutations in the TRR1, TRX1 and TRX2 genes was sporulated, and tetrad segregants representing the eight possible haplotypes were isolated and co-transformed with the pRS314PGK-p53 effector and pRS316-p53RE-LacZ reporter plasmids.