Table 4.
Chitotetraose coordination by CGL3
| Direct hydrogen bonds | ||
|---|---|---|
| DONOR | ACCEPTOR | Distance ± stddev (n=4) |
| Asn45 Nδ2 | O31 | 3.04 ± 0.15 |
| Arg64 Nη1 | O32 | 2.95 ± 0.06 |
| Arg64 Nη2 | O51 | 2.91 ± 0.12 |
| Asn73 Nδ2 | O61 | 3.01 ± 0.12 |
| Arg81 Nη2 | O33 | 3.00 ± 0.21* |
| Arg81 Nε | O62 | 2.82 ± 0.19 |
| Arg81 Nη2 | O43 | 3.02 ± 0.03* |
| O32 | Glu84 Oε1 | 2.52 ± 0.09 |
| O61 | Glu84 Oε1 | 2.67 ± 0.06 |
| Arg86 Nη1 | O32 | 2.94 ± 0.19 |
| Asn138 Nδ2 | O71 | 2.85 ± 0.12 |
| Water mediated hydrogen bonds | |
|---|---|
| Asn 47 Nδε2 | O31/O41* |
| Glu67 Oε2 | N22 |
| Arg81 N | O33/N21 |
| Ser134 Oγ | O41* |
| Lys136 N | O41* |
| Asn138 Oδ1 | O31 (O71/O41*) |
Distances are given in Å.
Numbering of chitotetraose atoms according to PDB Chemical Component Dictionary entry for chitotriose (CTO).
*
not observed in all four subunits.