. Author manuscript; available in PMC: 2009 Dec 2.

Published in final edited form as: J Mol Biol. 2008 Apr 3;379(1):146–159. doi: 10.1016/j.jmb.2008.03.062

Table 5.

Oligonucleotide primers

NdeI–CGL3N	5′–GGGGGGGGCATATGTTCCACATCCTCAGACTCG–3′
BamHI–CGL3C	5′–GGGGGGGGATCCTCAATTGGCGCGCGGCATGACG–3′
NdeI–His8–CGL3N	5′–GGGGGGGGCATATGGGCCATCATCATCATCATCATCATCACAGCGGCTTCCACATCCTCAGACTCGAATC–3′
CGL3–I43AN45A–fwd	5′–CAACTTGGGACCCACCGGGGCCGACGCCACCAACGTCAACTTGATCAAT–3′
CGL3–I43AN45A–rev	5′–ATTGATCAAGTTGACGTTGGTGGCGTCGGCCCCGGTGGGTCCCAAGTTG–3′
CGL3–R81W–fwd	5′–TACGGCGAAAGCTGGGGCCCTGAGGAACGAATTCC–3′
CGL3–R81W–rev	5′–TCGTTCCTCAGGGCCCCAGCTTTCGCCGTACGGGA–3′
CGL3–R81A–fwd	5′–GTATCCCGTACGGCGAAAGCGCGGGCCCTGAGGAACGAATTCC–3′
CGL3–R81A–rev	5′–GGAATTCGTTCCTCAGGGCCCGCGCTTTCGCCGTACGGGATAC–3′
CGL3–N138A–fwd	5′–GAGAAAGTGTCCTACAAGATCGCCGAAGGGCAGACCCCGCCT–3′
CGL3–N138A–rev	5′–AGGCGGGGTCTGCCCTTCGGCGATCTTGTAGGACACTTTCTC–3′
NdeI–CGL2N	5′–GGGGGGCATATGCTCTACCACCTCTTCGTCAACAA–3′
BamHI–CGL2C	5′–GGGGGGGGATCCCTAAGCAGGGGGAAGTGGGGGG–3′