Figure 2.
Time course of early LR development during the interaction of L. bicolor with mycorrhizal poplar and nonmycorrhizal Arabidopsis. A and B, Time course of LR development in poplar in response to L. bicolor (A) and in Arabidopsis (B). Beginning at 4 d (A) and 2 d (B) of poplar and Arabidopsis-L. bicolor interaction, a significant (Student's t test P < 0.05) LR stimulation was observed. LR stimulation was similar in indirect and direct contact in poplar. C, Effect of the polar auxin transport inhibitor NPA on LR increase in poplar (10 μm NPA) after 8 DOII and in Arabidopsis (5 μm NPA) after 6 DOII. LR ratio of plants in indirect contact versus control plants without fungus in the presence and absence of NPA is shown. LR stimulation was significantly (Student's t test P < 0.01) reduced by the NPA treatment. In each experiment and each condition, 10 to 15 individual poplar plants or 15 Arabidopsis seedlings were observed. Error bars indicate se. If not visible, they were smaller than the symbol at the data point.