Figure 1.

Assemblage of different genetic elements captured into entry clones via LR reaction to facilitate genetic studies in tomato. B1, B2, B4, L1, L2, L4, and R1: corresponding att Gateway recombination sites. Sp, spectinomycin; Sm, streptomycin; K_m and Kan, kanamycin bacterial and plant selectable markers, respectively; LB, left T-DNA border; RB, right T-DNA border; Prom, promoter; pdk-cat intron, pHELLSGATE12-derived intron spacer; ccdB, counter-selectable bacterial marker; and 3′OCS, octopine synthase terminator.