Figure 3.

Nonsymbiotic phenotypes of the enf1 mutant. A to F, Plants were grown for 21 d in vermiculite-filled pots supplied with B&D medium with or without 10 mm KNO₃. G, Plants were grown on B&D agar-solidified (1.5%, w/v) medium. A, MG20 (left) and enf1 (right) grown with no nitrogen. B, MG20 (left) and enf1 (right) grown with 10 mm KNO₃. C and D, Shoot length resulting from growth without KNO₃ (C) or with 10 mm KNO₃ (D). E and F, Number of true leaves per plant in medium without KNO₃ (E) or with 10 mm KNO₃ (F). G, Number of lateral roots per plant at 24 DAS. At least 30 plants were used in each experiment. Error bars indicate se, and statistical significance is indicated by asterisks (* P < 0.05, ** P < 0.01, by Student's t test).