Relative amounts of nitrogen fixation-related gene transcripts in enf1 nodules at 28 DAI. Transcript amounts of nifH, LjLbs, LjHb, Ign1, Sst1, and LjGlu1 were normalized against sigA (used as an internal control for nifH) and ATP synthase (internal control for LjLbs, LjHb, Ign1, Sst1, and LjGlu1) transcripts. The mean value of expression in wild-type plants was set to 1. The data represent averages ± se of three independent experiments of nodules derived from five different plants.