Figure 9.

NO production in nodules. A and B, Quantification of NO produced in nodules of MG20 and enf1 at 21 DAI (A) and 28 DAI (B). C, Quantification of NO in nodules that were treated with abamine. Nodules on the root of 28-d-old plants were treated with 20 μm abamine for 3 d. RFU values per nodule fresh weight at 515 nm, normalized against MG20 plants, are shown. The data represent averages ± se of three independent experiments derived from nodules of six to eight plants. In A and B, statistical significance is indicated by asterisks (** P < 0.01, by Student's t test). In C, the significance of differences among four groups was determined by the two-tailed multiple t test with Bonferroni correction following ANOVA (six comparisons in four groups), and the different letters refer to significant differences at P < 0.01.