Figure 3.

Recapitulation of the tld1-D phenotype. A, Wild-type (WT), 35S:TLD1-MYC, and 35S:TLD2-MYC T1 rice plants at the tillering stage. Reappearance of the tld1-D phenotype was observed only in the TLD1-overexpressing lines. B, Enlarged view of the TLD1 overexpressers in A, showing the enlarged third and fourth leaf angles in TLD1-3 as compared with the wild type. C, Statistics for the number of tillers in the wild-type and transgenic lines shown in A (n = 6). D, The overexpression of TLD1 but not TLD2 in Arabidopsis causes severe morphologic alterations. E, Immunoblot assay of TLD1-cMyc and TLD2-cMyc protein expression in rice and Arabidopsis. ABS, Amido Black staining of Rubisco (RBC) was used as a loading reference. [See online article for color version of this figure.]