Figure 6.

The effects of the UTRs of StBEL5 on the translation efficiency of GUS in tobacco protoplasts. A, Four constructs driven by the 35S promoter in pBI221 were analyzed: GUS + the 3′ UTR of StBEL5, the 5′ UTR of StBEL5 + GUS, GUS plus both UTRs attached, and the GUS cds alone. B, The LUC gene in pBI221 under the control of the CaMV 35S promoter was included as an internal control. A relative value for GUS expression was obtained by dividing the GUS activity by the specific LUC activity. Each transfection was performed three times. Data are means ± se. NOST, Nopaline synthase terminator. The levels of GUS translation have been adjusted to reflect differences in RNA accumulation for each of the constructs.