FIGURE 5.

The order of addition of BLM affects the efficiency of D-loop formation promoted by RAD51. A, the order of addition of individual components of the reaction mixtures. The numbers above the arrow indicate time of incubation. B, the D-loops were analyzed by electrophoresis in a 1% agarose gel. Note, migration of the D-loops was slightly changed in the presence of BLM, which probably indicates some conformational changes in plasmid DNA. C, the results from B presented as a graph. The error bars indicate S.E.