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. 2009 Jul 29;284(39):26964–26977. doi: 10.1074/jbc.M109.036293

FIGURE 2.

FIGURE 2.

Siva-1 binds SLIMMER in a yeast two-hybrid assay. AH109 yeast expressing the GAL4-DNA-binding domain (GAL4DBD) fused to the indicated LIM domains of FHL1, SLIMMER, KyoT2, or p53 (control) “baits” (A) were mated with Y187 yeast expressing GAL4-activation domain (GAL4AD) fused to Siva-1 or SV40-T (control) “preys” (B). C, transformants were plated onto media lacking tryptophan and leucine (−W−L), to select for diploid yeast expressing both bait and prey constructs and then recultured on media lacking tryptophan, leucine, adenine, and histidine and assessed for growth and LacZ activity (−4+X-α-gal). C-a, table summarizing the interaction of FHL1 bait proteins with either Siva-1 or SV40-T negative control. The presence of an interaction (+) was assessed by growth on minimal media lacking tryptophan, leucine, adenine, and histidine. No interaction is indicated by a minus sign (−). C-b, representative image of yeast growth on minimal media (lacking tryptophan, leucine, adenine, and histidine). Yeast expressing different combinations of bait and prey plasmids are indicated.