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. 2009 Nov 25;29(47):14779–14789. doi: 10.1523/JNEUROSCI.4161-09.2009

Figure 3.

Figure 3.

The proteasome complex is responsible for downregulation of CK2. The indicated concentrations of MG132 were injected intracerebroventricularly in mouse brains 1 h before tFCI in vivo (A) and were administered to a primary cortical neuronal culture subjected to OGD in vitro (B). Twenty hours after ischemia and reperfusion in vivo or 24 h of reoxygenation in vitro, Western blot was performed with a CK2α antibody and β-tubulin. C, A summary graph shows the dose-dependent effects of MG132 on restoration of the CK2α protein in an in vivo MCAO model (*p < 0.01 between sham and damaged samples without MG132; #p < 0.05 compared with damaged samples without MG132 after injury; n = 4). D, A summary graph shows that MG132 treatment at indicated concentrations with OGD restores the level of the CK2α protein (*p < 0.01 between control and OGD samples without MG132; #p < 0.05 compared with OGD samples without MG132; n = 6). I/R, Ischemic reperfusion; Con, control; O.D., optical density; R, reoxygenation. Error bars indicate SEM.