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RT-PCR analyses of NLY transcripts levels in PCB (stages 1–2), SCB (stages 1–3), DSB from an adult tree and 1-month-old seedlings and roots. PCR products were blotted onto nylon membranes and hybridized to 32P-labeled NLY cDNA clone. The rRNA fragment was amplified from the same RNA samples as a positive control.
