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. Author manuscript; available in PMC: 2010 Apr 3.
Published in final edited form as: J Mol Biol. 2009 Feb 10;387(3):570–583. doi: 10.1016/j.jmb.2009.01.068

Figure 3. Activity of RNase R H565T R572K and RNase R H456N H565T R572K on Structured RNA.

Figure 3

Assays were carried out as described under “Materials and Methods” with 10 μM ds17-A17 substrate and 2.5 μg wild-type RNase R, 10 μg H565T, 2.5 μg R572K, 2.5 μg H565T R572K and 5 μg H456N H565T R572K cell extract. Aliquots were taken at the indicated times and analyzed by denaturing PAGE. A schematic representation of the substrate is shown at the top of the figure with the position of the 32P label denoted by an asterisk.