. Author manuscript; available in PMC: 2010 Apr 3.

Published in final edited form as: J Mol Biol. 2009 Feb 10;387(3):570–583. doi: 10.1016/j.jmb.2009.01.068

Table 2. Comparison of the Activities of Wild-type RNase R, RNase R R572K and RNase R H456N H565T R572K.

Activity assays were performed as described under “Materials and Methods”. Each value represents the mean of at least three experiments.

Substrate	Activity (nmol min⁻¹ nmol⁻¹ RNase R)
	Wild-Type	RNase R	RNase R
	RNase R	R572K	H456N H565T R572K
ss17-A₁₇	5.3 ± 1.6	75 ± 24	27 ± 12
ds17-A₁₇	0.5 ± 0.2	5.1 ± 2.1	1.5 ± 0.4
A₁₇	40 ± 4.8	41 ± 7.0	33 ± 8.5
C₁₇	22 ± 13	130 ± 32	82 ± 28
U₁₇	42 ± 14	170 ± 65	87 ± 28
ss₁₇	29 ± 13	700 ± 99	200 ± 29