Table 3. RNA Binding to RNase R R572K.
The Kd was determined by a filter binding assay as described under “Materials and Methods”. Each value represents the mean of at least two experiments.
Substrate | Kd (nM) |
|
---|---|---|
Wild-Type RNase R | RNase R R572K | |
A17 | 0.8 ± 0.1 | 0.5 ± 0.1 |
A4 | 1,200 ± 90 | 5,800 ± 1,500 |