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. Author manuscript; available in PMC: 2010 Apr 3.
Published in final edited form as: J Mol Biol. 2009 Feb 10;387(3):570–583. doi: 10.1016/j.jmb.2009.01.068

Table 3. RNA Binding to RNase R R572K.

The Kd was determined by a filter binding assay as described under “Materials and Methods”. Each value represents the mean of at least two experiments.

Substrate Kd (nM)
Wild-Type RNase R RNase R R572K
A17 0.8 ± 0.1 0.5 ± 0.1
A4 1,200 ± 90 5,800 ± 1,500