Table 4. Site-Directed Mutagenesis Primers.

Nucleotides corresponding to the mutations are in bold. Complementary nucleotides between the forward and reverse primers are in italics. F = forward primer and R = reverse primer.

Mutant Primer	Primer Sequence
(273-279)	F: 5′ cgc gat tta ccg ctg gtc acc att gat agt gcc agc aca gaa gat atg gac gat gca gtt tac tgc gag aaa aaa cgc 3′
	R: 5′ gcg ttt ttt ctc gca gta aac tgc atc gtc cat atc ttc tgt gct ggc act atc aat ggt gac cag cgg taa atc gcg 3′
(428-433)	F: 5′ c cgt gaa gaa cgc ggt ggg atc ttt aaa gat cgc ccg gat gcg aag ttc att ttc aac gct gaa cgc cg 3′
	R: 5′ cg gcg ttc agc gtt gaa aat gaa ctt cgc atc cgg gcg atc ttt aaa gat ccc acc gcg ttc ttc acg g 3′
H456N	F: 5′ gaa cag acc cag cgt aac gac gcg aac aaa tta att gaa gag tgc atg att ctg gcg aat atc tcg gc 3′
	R: 5′ gc cga gat att cgc cag aat cat gca ctc ttc aat taa ttt gtt cgc gtc gtt acg ctg ggt ctg ttc 3′
(523/545)	F: 5′ g cgt gac tac gcg gag ctg cgc gag tcg gtt gcc gat cgt cct gat gca gaa atg 3′
	R: 5′ cc acg gtt ttc ttg atc gta aat cgc aaa gct ctg cga gcg gcg cag cat ggt atc cag cat ttc tgc atc agg acg atc g 3′
H565T	F: 5′ gc ctg gca ttg cag tcc tat gcg acc ttt act tcg ccg att cgt cgt tat cca gac3′
	R: 5′ gtc tgg ata acg acg aat cgg cga agt aaa ggt cgc ata gga ctg caa tgc cag gc 3′
R572K	F: 5′ gcg cac ttt act tcg ccg att cgt aaa tat cca gac ctg acg ctg cac cgc 3′
	R: 5′ gcg gtg cag cgt cag gtc tgg ata ttt acg aat cgg cga agt aaa gtg cgc 3′
H565T R572K	F: 5′ gc ctg gca ttg cag tcc tat gcg acc ttt act tcg ccg att cgt 3′
	R: 3′ cg gtg cag cgt cag gtc tgg ata ttt acg aat cgg cga agt aaa ggt cg 3′
(620-627)	F: 5′ ggt cag cac tgt tcg atg gcg cgc cgt ctc aac cgc atg gca g 3′
	R: 3′ ctt cag cca gtc agc cac atc gcg ttc tgc cat gcg gtt gag acg g 3′