Comparative analysis of HSCCC chromatograms of 500 mg of the crude sample of black currant, at different flow rates: (A) 1.0 mL/min, (B) 2.0 mL/min, (C) 1.5 mL/min. Two-phase solvent system: n-hexane/EtOAc/MeOH/H2O (5:15:4:7 v/v); stationary phase: upper organic phase; rotation speed: 850 rpm; detection wavelength: 254 nm; peaks I, II, III, IV corresponding to the following compounds: protocatechuic acid (1), caffeic acid (2), 4-hydroxybenzoic acid (3), myricetin (4), respectively.