Figure 10.

Electrochromatogram of the separation of a tryptic digest of cytochrome c using a long monolithic capillary column followed by collection of three fractions of eluent. Conditions: Poly(hexyl methacrylate-co-1,3-butenediol dimethacrylate-co-2-(acryloyloxy)ethyltrimethyl ammonium chloride) monolithic column, total length 72 cm, active length 64.5 cm, 100 µm i.d. Mobile phase: 30:70 v/v acetonitrile −40 mmol/L Tris phosphate buffer pH 2.5; Hydrodynamic injection 0.3 MPa, 90 s. Voltage: −30 kV. Detection at 200 nm.