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Electrochromatographic separation of tryptic digest of cytochrome C using monolithic capillary column. Conditions: Poly(hexyl methacrylate-co-1,3-butenediol dimethacrylate-co-2-(acryloyloxy) ethyltrimethyl ammonium chloride) monolithic column, total length 34.5 cm, active length 26 cm, 100 µm i.d. Mobile phase: 60:40 v/v acetonitrile −80 mmol/L Tris phosphate buffer pH 2.5; Hydrodynamic injection 0.4 MPa, 45 s; Voltage: −15 kV; EOF marker formamide. UV detection at 200 nm.
