| Final concentration | Volume | |
|---|---|---|
| GlutaMAX (200mM) | 24mM | 750μl |
| NaHCO3 (2.35 g/100ml) | 2.35mg/ml | 625 μl |
| MEM 10X | MEM 1X | 625 μl |
| Hepes (1M pH 7.6) | 20 mM | 125 μl |
| Collagen I | 2 mg/ml | 4.13ml |
| Total vol. | -------- | 6.255ml |
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