Imaging of Xenopus laevis hearts (stage 49) in vivo using OFDI. (a) to (d) High-speed OFDI 4-D imaging is presented through displacement color maps, revealing myocardial motion within a single cardiac cycle. The 900-fps imaging rate allows in vivo real-time 3-D imaging at a rate of 20 volumes per second (vps). (e), (f), and (g) High-resolution OFDI shows a series of frames of the atrioventricular valve (e) as it closes and [(g), valve marked by arrows] opens. Different anatomic structures within the heart can be clearly resolved, including (h) the ventricle with the trabeculae and [(j), spiral valve marked by arrow] the spiral valve within the TA. FFOCM images of the areas of the ventricle and the spiral valve are shown in (i) and [(k), spiral valve marked by arrow], respectively. v, ventricle; t, truncus arteriosus; a, atrium. Scale bars correspond to 100 µm.