Figure 2.

Example of student's digested phage DNA. This gel contains DNA from mycobacteriophage Puhltonio. Lanes 1 and 2 contain 1-kb standards and 0.5 μg of undigested phage DNA. Lanes 3–6 contain 0.5 μg of phage DNA digested with 10 U of the indicated restriction enzyme for approximately 2 h at 37°C. The gel is a 0.8% agarose submersible horizontal gel (7 × 10 cm) run in TAE buffer at 100 V and visualized with 0.5 μg/ml ethidium bromide. See Supplemental Material for additional gel images.