Fig. 2.

Stability of the EBNA1 and EBNA1P10A proteins. Cycloheximide (CHX) or DMSO was added to cells and aliquots were taken at various time points. The quantitative Western blot, probed with anti-EBNA1 mAb 1EB12 conjugated to Alexa Fluor 647, shows the persistence of both EBNA1 and EBNA1P10A proteins up to 48 h. A representative c-Myc Western blot is included to show that CHX did block translation. This experiment was repeated multiple times with similar results. There was 2-fold more lysate loaded for the BJAB8 sample to account for the difference in expression level.