TABLE 3.
Characteristics of P. aeruginosa LBM415 resistant mutants
| Strain | Description | LBM415 MIC (μg/ml) | Fold increase relative to parent |
|||
|---|---|---|---|---|---|---|
| mexX | mexY | mexZ | PA5471 | |||
| NB52019 | Wild type | 128 | ||||
| NB52021 | mexB | 64 | ||||
| NB52022 | mexX | 128 | ||||
| NB52023 | mexB mexX | 8 | ||||
| NB52020 | mexAB-oprM | 4 | ||||
| CDS1 | nfxB | >128 | ||||
| 14-bp deletion, base 92 | ||||||
| CDS6 | nfxB(A115C, T39P)b | >128 | ||||
| CDS28 | fmt; 16-bp deletion, position 833 | >1,024 | 13.5 | 18 | 8.4 | 13.9 |
| CDS28(pSW1)a | 128 | |||||
| CDS13 | mexAB-oprM fmt(C127T, R43C)b | >128 | 11 | 11.2 | 5.6 | 10.7 |
| CDS13(pSW1)a | 4 | |||||
| CDR1 | fmt::aacC1 | >1,024 | 8.3 | 7.2 | 4.9 | 6.5 |
| CDR2 | mexAB-oprM | >1,024 | 6.2 | 5.6 | 4.6 | 6.7 |
| fmt::aacC1 | ||||||
| CDS30 | folD(G493T, V165R)b | >1,024 | 5.6 | 8.9 | 5.4 | 4.4 |
| CDS30(pRC5)a | 128 | |||||
| CDS22 | mexAB-oprM folD(A424C, T142P)b | >128 | 3.5 | 4.8 | 3.3 | 4.7 |
| CDS22(pRC5)a | 4 | |||||
a
Plasmid pAK1900 alone did not alter susceptibility.
b
Nucleotide and amino acid changes are shown in parentheses.