FIG. 6.

Effects of HA and derivatives on in vitro transcription-translation. (A) Detection of template-dependent synthesis of GFP by using fluorescence and native polyacrylamide gel electrophoresis in the presence of template with increasing concentrations (μM) of the antibiotic HA, DHHA, and compound 7 on GFP synthesis; (B) quantitation of effects of HA, DHHA, and compound 7 on GFP synthesis; (C) quantitation of effects of HA and compounds 3, 4, 5, and 6 on GFP synthesis. The experiments whose results are shown in panels B and C were performed in triplicate. GFP fluorescence is given as a percentage, where 100% is defined as the fluorescence detected in the absence of the antibiotic. Rel., relative.