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. 2009 Oct 5;53(12):5108–5113. doi: 10.1128/AAC.00505-09

FIG. 6.

FIG. 6.

Parasitological evaluation of infected BALB/c mice treated with amiodarone plus miltefosine by a Leishmania multiplex PCR assay. PCRs were performed as described in Materials and Methods. Lane 1, molecular weight marker (1 Kb Plus; Invitrogen); lane 2, PCR mixture without DNA (negative control); lane 3, L. mexicana genomic DNA (positive control); lane 4, untreated lesion DNA from infected mouse footpad; lanes 5 to 14, DNA from lesions of infected mouse footpads treated with 50 mg/kg/day amiodarone plus 20 mg/kg/day miltefosine for 49 days; lane 15, genomic DNA from a healthy footpad. Arrows indicate the β-tubulin amplification products (900 bp) and the specific fragment from L. mexicana amplified with the F1 and B2 primers (260 bp). The absence of a 260-bp band indicates the absence of Leishmania in the lesion.