TABLE 1.
Susceptibility of PM-KO lines to known protease inhibitors
| Parasite line | IC50a |
||||
|---|---|---|---|---|---|
| E-64 (μM) | ALLN (nM) | Leupeptin (μM) | Ro 40-4388 (nM) | Pepstatin A new (μM) | |
| Dd2 | 4.5 ± 0.6 | 104.7 ± 13.5 | 7.5 ± 0.6 | 208.8 ± 16.1 | 25.0 ± 3.0 |
| Δpfpm1 | 3.7 ± 0.2 | 94.5 ± 9.6 | 6.8 ± 0.6 | 197.9 ± 16.0 | 27.5 ± 2.1 |
| Δpfpm2 | 3.0 ± 0.2*** | 86.4 ± 9.8 | 5.2 ± 0.4 | 178.6 ± 12.7 | 23.5 ± 1.3 |
| Δpfpm3 | 2.8 ± 0.1*** | 61.8 ± 7.8* | 4.4 ± 0.7** | 180.9 ± 14.3 | 20.0 ± 3.7 |
| Δpfpm4 | 3.0 ± 0.1** | 84.5 ± 14.3 | 6.7 ± 0.7 | 201.8 ± 18.5 | 23.4 ± 2.6 |
| GC03 | 3.2 ± 0.2** | 97.9 ± 10.3 | 10.1 ± 0.7* | 162.4 ± 11.7 | 36.0 ± 2.8* |
| 3D7 | 3.4 ± 0.2 | 72.4 ± 7.8 | 6.8 ± 0.2 | 187.1 ± 9.4 | 16.4 ± 1.1 |
| Δ3pfpm | 3.0 ± 0.2 | 44.1 ± 7.9* | 17.3 ± 2.5* | 175.6 ± 11.0 | 11.5 ± 1.3* |
| Δ4pfpm | 2.1 ± 0.2*** | 7.9 ± 0.7*** | 7.8 ± 1.4 | 187.6 ± 11.6 | 18.4 ± 1.3 |
a
IC50s are expressed as means ± SEMs. Each value was determined from four to nine separate drug assays performed in duplicate. The degree of statistical significance is indicated with an asterisk(s): *, P < 0.05; **, P < 0.01; ***, P < 0.001.