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Primers used
| Target gene | Primer | Sequence (5′ to 3′) |
|---|---|---|
| sdeB | SdeB1Eco | CGGAATTCTTGGCAAACGGCGATCGGGTG |
| SdeB4Hin | CCGAAGCTTGCTGCGCAAGAGGCTGAAACC | |
| xylE | XylE1Pst | GTCCTGCAGCTGGCCCTGGCCTGTGAAGTG |
| XylE4Bam | AGGATCCCAGCTTGCCGCTGGCCGAAC | |
| Transposon | KAN-2GWF1 | AGAGCATTACGCTGACTTGACG |
| KAN-2GWR1 | GATAGATTGTCGCACCTGATTGC | |
| hasF | HASF1 | CAGAAGCTTGGCACATTCAGCCACCTGATG |
| HASF2 | CAGGAATTCGACCCCGTGTTTATCAACGTG |
