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. 2009 Oct 5;53(12):5155–5162. doi: 10.1128/AAC.00532-09

FIG. 2.

FIG. 2.

Effect of subinhibitory concentrations of ofloxacin and moxifloxacin on the production of protease Cwp84 in three moxifloxacin-resistant NAP1/027 strains (6296, 6425, and CD07-259), a moxifloxacin-susceptible NAP1/027 strain (CD196), a non-NAP1/027 strain (ATCC 43603), and the two in vitro-selected moxifloxacin-resistant mutants (CD196-M1 and ATCC 43603-M1). Immunoblots and densitometry analyses of glycine extracts were performed. Anti-Cwp84 antibodies reacted with a unique band of 85-kDa protein. Histograms show mean relative optical density ratios (optical density of extract from bacteria grown with antibiotic versus optical density of extract from control grown in antibiotic-free medium) ± standard deviations; optical densities were determined by densitometry scanning. The inserts show examples of Cwp84 immunoblots for each strain grown in the absence (C, control without antibiotic) or presence of 0.5× MIC of ofloxacin (OFX) or moxifloxacin (MXF).