FIG. 1.

Induction of CAMP mRNA expression by PBA. (A) VA10 cells were stimulated with 4 mM PBA or treated with solvent alone and harvested after the indicated period of time. (B) VA10 cells were stimulated with the indicated concentrations of PBA or solvent (Control) for 24 h. These experiments were performed once for initial assessment. CAMP mRNA levels were determined by real-time RT-PCR. Individual samples were normalized to total RNA input. Results were normalized to expression in control samples, where controls were given the arbitrary value of 1.